![]() ![]() cerevisiae cells with lower and variable levels of ATP, proteins stick together, forming clumps. identified mutants of the yeast Saccharomyces cerevisiae that had low levels of ATP, and studied how these cells differ from normal yeast The results showed that, in S. To answer these questions, Takaine et al. It is unclear why cells keep so much ATP, or whether this excess ATP has any other purpose. Cells just need enough ATP to survive, but most cells store a lot more than they need. Like a battery, each ATP molecule contains a specific amount of energy that can be released when needed. ![]() Editor's evaluationĬells use a chemical called adenosine triphosphate (ATP) as a controllable source of energy. The present study demonstrates that cellular ATP homeostasis ensures proteostasis and revealed that suppressing the high volatility of cellular ATP levels prevented cytotoxic protein aggregation, implying that AMPK and ADK are important factors that prevent proteinopathies, such as neurodegenerative diseases. Moreover, pharmacological elevations in ATP levels in an ATP-reduced mutant prevented the accumulation of α-synuclein aggregates and its cytotoxicity. Single-cell imaging of ATP-reduced yeast mutants revealed that ATP levels in these mutants underwent stochastic and transient depletion, which promoted the cytotoxic aggregation of endogenous proteins and pathogenic proteins, such as huntingtin and α-synuclein. We herein demonstrated that AMP-activated protein kinase (AMPK) and adenylate kinase (ADK) cooperated to maintain cellular ATP levels regardless of glucose levels. However, the significance of this high ATP level under physiological conditions and the mechanisms that maintain ATP remain unclear. ![]() Adenosine triphosphate (ATP) at millimolar levels has recently been implicated in the solubilization of cellular proteins. ![]()
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